KESIMPULAN UNTUK KAJIAN KLINIKAL
Kajian Kevitalan Ekstarak Sel induk PhytoCellTec
*how can wew show our PhytoCellTec product improve vitality of human stem cell?
*Stimulation of growth or over-expression of markers are not good parameter for the activity of stem cells
*Colony forming efficiency ( CFE )
*Special cell cultures enriched in epidermal stem cells are seeded at low density.The number of colonies formed is a value for the number of vital stem cells
Peningkatan Pembentukan Kolon yang efisyen dengan Sel induk dari Epal
Stimulation of CFE by 92% with
PhytoCellTecTM Malus Domestica
Perlindungan keatas Sel Induk Epidermis dari Sinaran UV dengan Sel Induk dari Anggur
*Stimulation of CFE by 86% with PhytoCellTecTM Solar Vitis
*58% decrease of CFE after exposure to UV
-> with PhytoCellTecTM Solar Vitis stem cells were fully protected stem cells were fully protected against negative effect of UV
Kesan Ekstrak Sel Induk Malus Domestica,Keatas Sel Induk Manusia
Dr.M.Siebar
BIONET Corp..Taipei 231 Taiwan
Sponsor
Mibelle AG - Biochemistry
5033 Buchs Switzerland
Test Product
* Malus Domestica stem cell extract
Cell Line
* Umbicical cord derived stem cells
Assays
* Growth / Proliferation test
cells were seeded on 6-well plates ( 2x10 4/well ).The medium was changed after a first incubation period,the cell number was counted and pictures were taken
*UV Exposure Damage Test
cells were seeded on 96-well plates ( 5x10 3/well ),the medium was changed after a first incubation period and the cells were exposed to UV radiation afterwards. The MTS assay was performed after 48 hours.
Result
Malus Domestica stem cell extract shows a promoting effect on stem cell growth.Its also shows a protective effect against UV damage.
Kesan dari PhytoCellTecTM Malus Domestica keatas folikal rambut manusia
F.Juchaux
BIOalternatives,86160 Gencay,France
Sponsor:
Mibelle AG - Biochemistry
5033 Buchs,Switzerland
Introduction
hair grows in cycles.String with anagen ( The growth phase ), and finally exogen ( shedding phase ).The hair follicles used in the assays show a growth phase ( anagen ) of 16 days before they switch to catagen.
Test Product
* 0.2% PhytoCellTecTM Malus Domestica filter sterilized
Biological Model
* Hair bulbs micro dissected from human skin fragments.
Assays
* Hair Follicle Growth
Hair bilbs were cultivated in William Culture medium containing or not ( control ) 0.2% PhytoCellTecTM Malus Domestica.The hair follicle growth was followed during 20 days with length measurements and photographs taken.
Result
At a concentration of 0.2% PhytoCellTecTM Malus Domestica slowed down hair follicle growth by 50% compared to the control.
Kesa Anti-Kedutan oleh PhytoCellTecTm Malus Domestica
Dr.H.P.Nissen
Derma Consult GmbH,53347 Alfter,Germany
Sponsor:
Mibelle AG - Biochemistry
5033 Buchs,Switzerland
Test Product
* Cream with 2% PhytoCellTecTM Malus Domestica
Test Area
* Crow's feet
Volunteers
* Number of individuals:20
Age : 37-64
Sex : Female
Application
*Duration : 28 days
*Frequency : twice daily
Study design
* Day 0
determination of the parameters in the test areas,first application of the test product
* Day 1 to 3
Application of the test product twice a day
* Day 14
Determination of the test parameter 8-12h after the last daily test product application
* Day 15 to 27
Application of the best product twice day
* Day 28
Determination of the test parameter 8-12h after the last daily test product application
Test Parameters
Wrinkle dept : by means of PRIMOS 5.5 ( GFMesstechnik GmbH,Tetlow,Germany )
Result
Two daily applications of the test cream containing 2% PhytoCellTecTM malus Domestica over 28 days resulted in a significant decrease in wrinkle depth in all of volunteers tested.
Result
Two daily applications of the test cream containing 2% PhytoCellTecTM Malus Domestica over 28 days resulted in a significant decrease in wrinkle depth in all volunteers tested.
Kesan oleh PhytoCellTecTM Malus Domestica,Sel Progenitor Epidermis dalam bentuk 3D Model Kulit
Cell Culture Reasearch Institute
Switzerland
Sponsor :
Mibelle AG - Biochemistry
5033 Bushs,Switzerland
Introduction
In a pevious experiment ( S-234 ) it could be shown that Malus domestica stem cell extract of epidermal progenitor cells. In this experiment this effect is further investigated using 3D models.
Test product
* Malus domestica stem cell culture extract 0.01%
Cells
* Human keratinocyte progenitor cells
3D Model
Primary keratinocytes are cultured in a medium specially designed to mimick the microviroment of the adult stem cell niche. This medium ( M1 ) enchances the growth of progenitor cell.After incubation.M1 is replace by M2, a medium that together with primary human keratinocytes establishes 3D epidermal models which accurately represent the in vivo structure
Assay
Early passage ( "New" ) keratinovyte progenitor cells and late passage ( "Old" ) cells are cultured in the presence or absence of 0.01% Malus domestica stem cell extract in order to see if "old" cellsmaintain the ability to build 3D models if they were cultured in 0.01% Malus domestica stem cell extract.
Result
The result show that 0.01% Malus domestica stem cell extract significantly increases the ability of late passage keratinoscytes to esthabish 3D models.whereas-treated late passage cells formed a model which was indistinguishable from the control cells.
3D MODEL
Kajian terhadap Beta-1,3-Glucan
Indikasi
* Mempercepatkan proses penyembuhan luka
* Meingkatkan keupayaan imun terhadap bakteria
* Merangsang tindak balas imun dalam badan
Penerbitan ( semenjak 10 tahun yang lalu )
* Lebih daripada 40 paten
* Lebih daripada 1000 kertas kerja
Penindasan Defisiensi Imun
* Sinar UV mendorong penindasan defisiensi terhadap aktiviti imunologi di dalam epidermis
* Kedayahidupan sel berkompeten imuno ( iaitu sel Kerotinocytes dan Langerhans ) terjejas
* Penindasan defiensi imun menyokong pengembangbiakan kanser
RINGKASAN UJIAN KLINIKAL KE ATAS PROLIBRA
Ujian Klinikal ke Atas Manusia 2
Jumlah jisim lemak jelas berkurangan dalam kumpulan Prolibra-pengurangan sebanyak 5.81% berbanding dengan kumpulan placebo,yang hanya mengalami pengurangan sebanyak 2.29% ( p <- 0.01,rajah 1 ).L.Ward; E. Bastian: Dairy Components in Weight Management: A Broad Perspective. Functional Dairy Product ( Volume 2, Chapter 1 ). Woodwhead Publishing Limited,Cambridge,England,2007
Ujian Klinikal ke Atas Manusia 2
Kehilangan berat badan sebanyak 79% oleh orang yang mengambil Prolibra adalah lemak lwn.51% bagi orang yang mengambil bahan kawalan ( p<-0.01 ),Rajah 2 ).Frestedt,J;.Zenk, J; Kuskowski,M; Ward, L;Bastian,E; A whey-protien supplement increase fat loss and spares lean muscle in obese subject: A randomized human clinical study. Nutrion & Metabolism,2008 5:8
Ujian Klinikal ke Atas Manusia 3
Para peserta di bawah kajian mengalami statistik pengurangan ( p <- 0.05,Rajah 3 )apabila Prolibra dicampurkan kepada cecair makanan.Determanation of Glycemic Index potential of Prolibra.2007. Laporan daripada Index Laboratories
Ringkasan
Prolibra merupakan satu pendekatan dari mengawal berat badan. ketiga-tiga ujian telah menentukan secara klinikal bahawa Prolibra mampu mengurangkan berat badan sambil memerihara berat badan yang unggul dan seterusnya mengurangkan glisemia selepas makan.
